HPLC FAQ
Q Why I am getting split peaks on my chromatograms?
A Split Peaks
What you expect What you got 
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Possible Cause
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Remedy
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Contamination of column or guard
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Remove guard column. If the problem is solved replace it. If not the problem is most likely due to a blocked column frit which may need replacing. Otherwise replace the analytical column.
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Sample Solvent incompatible with mobile phase
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Change solvent used in sample preparation. This will ideally be the mobile phase.
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Q Why I do I have a noisy baseline on my chromatograms?
A Base line noise (Irregular)
What you expect  What you got 
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Possible Cause
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Remedy
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Leak
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Check for any evidence of leaking and fix as appropriate.
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Poor quality mobile phase
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Use HPLC grade solvents
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Cell contaminant
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Clean detector cell
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Weak lamp
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Replace detector lamp
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Air Bubbles in detector
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Purge Detector
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HPLC 
