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Re:HPLC column - 2006/06/12 18:33
SGE HPLC fittings, guard cartridges and in-line filters are completely compatible with all other manufacturers' HPLC columns and systems.
If you are doing any method development with reversed phase HPLC, Wakosil II 5C18 RS columns should be the first columns to try.
Always use a guard cartridge to prolong column life.
For quick and easy column connections use EasyLOK nuts.
If your reversed phase HPLC run is taking too long on a C18 or ODS column try using a SGE Wakosil II 5C8 RS (basic compounds and method development), Exsil C8 (existing method on an older style ODS), or Nucleosil C8 (protein analysis and peptides).
SGE Guard cartridges can be used in conjunction with competitors' columns (for those customers with methods validated on other columns).
PEEKsil tubing is an excellent alternative to PEEK tubing as its internal bore is unaffected by any organic solvents.
Wakosil II 5C18 AR columns have a pH range of 1.4 - 9.4 making it a very TOUGH phase.
If you are close to the limit of detection using a standard 4.6mm or 4mm ID HPLC column try using a 2mm ID column. This saves solvent too.
For complete loop fill, the syringe capacity should be greater than twice the loop volume. The loop capacity sets the injection volume. For partial loop fill, the injection volume should be no greater than half the loop capacity. The injection size sets the injection volume.
If you can't pre-filter samples, make sure to use a low dead volume in-line filter after the injector.
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