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HPLC column - 2005/11/28 19:11
How you clean a column, to a great extent depends on what you are trying to clean off of it. I have seen some chemicals used that I would have been reluctant to try on my own for fear they would damage the column. One must also consider the column itself, substrate and C-18 bonding. A silica substrate without end capping versus, non-polar end capping versus the new polar end capping columns. There are also C18 columns with substrates other than silica. The simplest most strate forward cleaning that I use for most of my reverse phase columns is with a 3 solvent gradient HPLC. I use a program with the following parameters. Start at 33%H2O:33%MeOH:34%MeCN flowing at 1ml/min. Over 30 minute periods change to 100% H2O, then to 100% MeOH and finally to 100% MeCN. Then over a 15 minute period return to starting conditions. Set the instrument to run this program 3 times overnight.
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