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Loss of resolution through heating. - 2005/11/28 19:04
I have encountered different opinions concerning buffered mobile phase pH. One side says to make the buffer solution to the required strength and pH and then add to the organic. The other side says to make the buffer solution to the required strength and add to the organic; then, you adjust the pH. Which is the proper procedure? It would appear to me that you would have to adjust the buffer first. Thanks for any input.
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